Distribution of single-channel open times in WT and Q266H GlyRs
| Open Times | Mean ± S.E.M. | τ1 | τ2 | τ3 | ||||
|---|---|---|---|---|---|---|---|---|
| ms | ms | % | ms | % | ms | % | ||
| WT | 4.75 ± 0.86 | 0.42 ± 0.19 | 42 ± 11 | 2.42 ± 0.76 | 42 ± 7.3 | 14.7 ± 3.02 | 20 ± 3.6 | |
| Q266H | 0.98 ± 0.12* | 0.16 ± 0.09 | 52 ± 20 | 0.52 ± 0.17 | 37 ± 16 | 3.26 ± 1.13* | 12 ± 6.1 | |
Open-time distribution of the main conductance state in single-channel openings in WT and Q266H GlyRs recorded in outside-out membrane patches held at −60 mV in response to low doses of glycine. The distribution of open times was fitted with three exponential components. τ1, τ2, and τ3 refer to the mean time constants of these exponential components and the percent refers to their relative contribution to the total channel open-time distribution. From the individual time constants and their relative contributions, the mean open time was calculated. All data represent mean and S.E.M. from seven WT and four Q266H GlyR patches. An unpaired t test was used to compare the amplitude and relative contributions of the three components and the mean open time between WT and Q266H. Significant differences (p < 0.05) between WT and Q266H are indicated by an asterisk.