Effect of DENSPM SSAT activity and polyamine pools in nontransgenic and transgenic animals2-a
| Tissue/treatment | SSAT Activity (pmol/mgprotein/min) | Polyamine pools (pmol/mg tissue wet weight) | ||||
|---|---|---|---|---|---|---|
| (pmol/mg tissue wet weight) | ||||||
| Put | Ac-Spd | Spd | Spm | DENSPM | ||
| Brain (tg−) | 11 ± 1 | <5 | <5 | 274 ± 38 | 240 ± 10 | |
| Brain (tg−) + DENSPM | 11 ± 3 | 16 ± 5 | <5 | 329 ± 61 | 220 ± 17 | 13 ± 4 |
| Brain (tg+) | 18 ± 4 | 173 ± 93* | 13 ± 5* | 219 ± 23 | 311 ± 28* | |
| Brain (tg+) + DENSPM | 74 (95,53) | 285 (323,246) | 17 (19,15) | 189 (208,169) | 285 (264,306) | 19 (20,18) |
| Intestine (tg−) | 21 ± 5 | 33 ± 9 | <5 | 1031 ± 183 | 583 ± 126 | |
| Intestine (tg−) + DENSPM | 33 ± 35 | 99 ± 29 | <5 | 934 ± 179 | 490 ± 90 | 230 ± 74 |
| Intestine (tg+) | 24 ± 16 | 1095 ± 332** | 42 ± 3*** | 800 ± 186 | 709 ± 112 | |
| Intestine (tg+) + DENSPM | 593 (686,499) | 1474 (1475,1473) | 45 (48,41) | 86 (88,83) | 172 (177,167) | 841 (915,767) |
| Skin (tg+) | n.d. | 413 ± 131 | 40 ± 4 | 372 ± 27 | 237 ± 29 | |
| Skin (tg+) + DENSPM | n.d. | 1000 (939,1060) | 89 (74,104) | 100 (64,136) | 78 (47,109) | 348 (367,329) |
↵2-a For experimental details see Table 1. In skin, the statistical significances refer to the treatment effect. n.d., not determined.