Table 5

Relative expression of CYP1A1 and CYP1B1 mRNA in Hepa-1 cell lines after treatment with TCDD or BA for 18 h

Cell Line	Treatment	CYP1A1^5-a	CYP1B1^5-a
WT	Control	1^5-b	1^5-b
	TCDD	179 ± 31	20 ± 2.5
	BA	170 ± 17.3	19 ± 3.7
LA1	Control	0.8 ± 0.4	0.5 ± 0.2
	TCDD	115 ± 26	16 ± 1.6
	BA	111 ± 18	15 ± 3.4
LA2	Control	0.6 ± 0.2	17 ± 2.4
	TCDD	0.5 ± 0.2	15 ± 1.8
	BA	0.4 ± 0.3	16 ± 2.2

↵5-a Mean and S.D. of three PCR determinations. Each determination was obtained by quantifying gel images such as in Fig.4B, using standard curves similar to the one in Fig. 1. All values were normalized to GAPDH expression levels.
↵5-b Relative to control WT, which is defined as 1 for either CYP1A1 or CYP1B1.