Binding profile of wild-type CB2 and K109A and K109AS112G mutant cell lines
| Ligand | Ki | ||
|---|---|---|---|
| CB2 | K109A | K109AS112G | |
| nM | nM | nM | |
| Δ9-THC | 44.9 ± 21.8 | 32.6 ± 0.78 | NSB2-a |
| Cannabinol | 92.7 ± 18.42-a | 200.7 ± 28.92-a | NSB2-a |
| CP-55,940 | 0.75 ± 0.08 | 0.82 ± 0.23 | NSB2-a |
| JWH015 | 13.8 ± 4.6 | 140 ± 3.4* | 266 ± 812-a * |
| WIN 55,212-2 | 0.91 ± 0.14 | 1.30 ± 0.14 | 3.01 ± 1.102-a |
| Anandamide | 306 ± 48 | 153 ± 2.5 | NSB2-a |
Inhibition constants were obtained from competition experiments using [3H]CP-55,940 or [3H]WIN 55,212-22-a in stably transfected HEK-293 cells (see Experimental Procedures). Data are means ± S.E.M. of three independent experiments, each performed in triplicate.
↵2-a NSB, no specific binding. * Statistically significant differences from control (p < .05).