Comparison of EC50 values and maximal responses for histamine-induced inositol phosphates accumulation in CHO cells expressing wild-type or mutant H1R.
| Receptor | Control EC50 | PMA EC50 | Staurosporine EC50 | |||
|---|---|---|---|---|---|---|
| μM | fold | μM | fold | μM | fold | |
| Wild-type | 2.95 ± 0.19 | 10.6 ± 1.5 | 29.04 ± 1.88 | 4.1 ± 0.2 | 1.57 ± 0.09 | 10.2 ± 1.1 |
| S396A | 3.38 ± 0.25 | 10.1 ± 0.7 | 20.20 ± 1.45 | 5.3 ± 0.5 | ||
| S398A | 0.85 ± 0.10 | 10.2 ± 0.7 | 1.71 ± 0.19 | 8.2 ± 0.6 | ||
| S396A/S398A | 0.79 ± 0.06 | 9.8 ± 0.3 | 1.10 ± 0.08 | 8.0 ± 0.2 | 1.10 ± 0.10 | 9.0 ± 0.7 |
EC50 values and maximal responses (fold) for histamine-induced inositol phosphates accumulation were determined from results of Fig. 5and other experiments, which were performed in the presence of 1 μM staurosporine without PMA. Results represent the mean ± standard error of three independent experiments.