Effect of DENSPM on polyamine enzyme activities and pools in nontransgenic and transgenic animals1-a
| Tissue/treatment | Enzyme activity | Polyamine pools (pmol/mg tissue wet weight) | ||||||
|---|---|---|---|---|---|---|---|---|
| ODC (pmol/mgprotein/h) | AdoMetDC (pmol/mgprotein/h) | SSAT (pmol/mgprotein/min) | Put | Ac-Spd | Spd | Spm | DENSPM | |
| Liver (tg−) | 9 ± 5 | 692 ± 288 | 8 ± 0.4 | 16 ± 6 | <5 | 942 ± 79 | 930 ± 58 | |
| Liver (tg−) + DENSPM | 88 ± 73 | 875 ± 292 | 12 ± 3 | 167 ± 94 | 6 ± 1 | 592 ± 22 | 671 ± 87 | 467 ± 90 |
| Liver (tg+) | 180 ± 20* | 813 ± 142 | 20 ± 7* | 543 ± 190* | 62 ± 12*** | 870 ± 105 | 420 ± 38*** | |
| Liver (tg+) + DENSPM | 439 (503,374) | 558 (546,569) | 16670 (21368,11978) | 859 (632,1086) | 37 (27,48) | 12 (12,12) | 10 (15,4) | 2225 (2108,2341) |
| Spleen (tg−) | 0.5 ± 0.2 | 19 ± 5 | 43 ± 4 | 87 ± 14 | <5 | 1226 ± 88 | 921 ± 95 | |
| Spleen (tg−) + DENSPM | 6 ± 4 | 23 ± 3 | 82 ± 44 | 282 ± 111 | 9 ± 3 | 759 ± 164 | 623 ± 129 | 295 ± 138 |
| Spleen (tg+) | 23 ± 7 | 57 ± 15* | 84 ± 21* | 2760 ± 517* | 221 ± 87* | 811 ± 126** | 669 ± 64* | |
| Spleen (tg+) + DENSPM | 123 (17,228) | 51 (38,63) | 3240 (3808,2673) | 4085 (3720,4451) | 328 (367,288) | 182 (135,228) | 144 (138,150) | 795 (921,669) |
↵1-a The animals received 125 mg/kg of diethylnorspermine on four consecutive days and were euthanized on the fifth day. There were initially three animals in control groups and four animals in the treatment groups, but only two survived of the transgenic group receiving the drug. Means ± standard deviations. * P < .05; ** P < .01; *** P < .001 (refers to the statistical differences between untreated nontransgenic and transgenic groups). Put, putrescine; Ac-Spd, N1-acetylspermidine; Spd, spermidine; Spm, spermine; tg−, nontransgenic; tg+, transgenic.