Key parameters of channels analyzed in Figs. 8 and 9, A, and B
| Agonist (μM) | Fig.8 | Fig. 9A | Fig. 9B | |||
|---|---|---|---|---|---|---|
| cGMP 5 | cAMP 250 | cGMP 5 | cAMP 100 | cGMP 5 | cAMP 250 | |
| Duration (s) | 68 | 328 | 122 | 52 | 216 | 146 |
| No. events | 1080 | 4925 | 1574 | 924 | 2071 | 2121 |
| Popen (%) (mean ± S.D.) | 26.9 ± 8.3 | 20.6 ± 6.6 | 51.5 ± 16.5 | 69.6 ± 15.7 | 56.8 ± 20.3 | 78 ± 8.7 |
| τc1(Ac1) | 0.35 (10%) | 0.47 (5%) | 0.81 (50%) | 0.58 (20%) | 0.84 (39%) | 0.59 (26%) |
| τc2(Ac2)* | 89 (90%) | 106 (95%) | 127 (50%) | 39 (80%) | 116 (61%) | 33 (74%) |
| τo1(Ao1) | 33 (100%) | 27 (100%) | 1.7 (22%) | 0.35 (4%) | 2.4 (22%) | 104 (100%) |
| τo2(Ao2)* | 103 (78%) | 72 (96%) | 143 (78%) | |||
| Log of likelihood | 2019 | 9465 | 3255 | 1833 | 3518 | 4061 |
| L 1 * | 6.5 × 10−3 | 5.5 × 10−4 | 5.3 × 10−3 | |||
| L 2 | 3.1 | 1.5 | 47 | 19 | 55 | 20 |
| K 1(μM)* | 4.8 | 4 | 0.52 | |||
| K 2(μM) | 38 | 1157 | 110 | 656 | 182 | 1124 |
Abbreviations: τ, time constant of an exponential component in milliseconds; A, fractional area of an exponential component; c, closed times; o, open times; 1 and 2, first or second component; *, “where applicable”, some channels lack a second component in histogram;K, equilibrium dissociation constant; L, equilibrium gating constant, equals opening rate constant divided by closing rate constant.