Effect of NQ on the association kinetic of [3H]ryanodine with SR
| Control | NQ, 0.5 μM | NQ, 5 μM | |
|---|---|---|---|
| k obs(min−1) | 0.0172 | 0.0430 | 0.0559 |
| T 1/2 (min) | 40.3 | 16.1 | 12.4 |
| B eq(pmol/mg) | 0.140 | 0.428 | 0.254 |
SR membranes (50 μg/ml) were incubated in the absence or presence of NQ as described in Fig. 6B. Data were analyzed by nonlinear regression analysis (ENZFITTER, Elsevier Biosoft). Specific [3H]ryanodine binding as a function of incubation time was fit with an exponential, from which equilibrium or maximal binding (Beq), apparent association rate constant (kobs), and apparent half-time (T1/2) were calculated. The values are the average of two experiments, each performed in duplicate.