cAMP | cGMP | |||
---|---|---|---|---|
EC50 ± S.E.M. (n) | nH ± S.E.M. (n) | EC50 ± S.E.M. (n) | nH ± S.E.M. (n) | |
rOCNC1 | ||||
Wild-type | 85 ± 7 (18) | 2.0 ± 0.1 (18) | 2.6 ± 0.2 (14) | 2.1 ± 0.1 (14) |
Y512F | 188 ± 26 (6) | 2.4 ± 0.1 (6) | 2.8 ± 0.4 (5) | 1.9 ± 0.4 (5) |
Y512A | 83 ± 11 (7)* | 2.1 ± 0.1 (7) | 1.5 ± 0.1 (2) | 2.1 ± 0.2 (2) |
Y512S | 74 ± 18 (6)* | 1.9 ± 0.1 (6) | 2.7 ± 0.4 (3) | 1.9 ± 0.1 (3) |
Y512D | 157 ± 18 (2) | 1.7 ± 0.1 (2) | 5.2 ± 0.2 (3) | 2.3 ± 0.2 (3) |
F477A | 167 ± 25 (2) | 1.8 ± 0.1 (2) | 2.5 ± 0.9 (2) | 2.0 ± 0.2 (2) |
Y482W | 2.9 (1) | 1.4 | ||
Y547A | 85 ± 40 (2) | 1.8 ± 0.5 (2) | 5.7 (1) | 1.7 |
F551W | 1 (1) | 3.5 | ||
Y565A | 9.5 ± 1.1 (10) | 2.2 ± 0.3 (10) | 0.33 ± 0.07 (2) | 2.0 ± 0.5 (5) |
Y565L | 30 ± 3 (5) | 2.0 ± 0.1 (5) | 0.8 ± 0.1 (2) | 2.1 ± 0.1 (2) |
Y565D | 34 ± 5 (7) | 1.8 ± 0.2 (7) | 1.1 ± 0.3 (5) | 2.0 ± 0.2 (5) |
Y565Δ | 69 ± 3 (3) | 2.1 ± 0.4 (3) | 2.5 ± 0.6 (3) | 1.5 ± 0.1 (3) |
Brcng | ||||
Wild-type | 128 ± 8.7 (11) | 2.2 ± 0.1 (11) | ||
F533Y | 143 ± 11 (4) | 2.3 ± 0.1 (4) | ||
F533A | 126 ± 14 (4) | 2.1 ± 0.1 (4) | ||
F533D | 127 ± 26 (3) | 2.0 ± 0.1 (3) | ||
Y586F | 39 ± 2.0 (2) | 3.4 ± 0.2 (2) | ||
Y586W | 77 ± 11 (2) | 2.0 ± 0.4 (2) |
* For the batches of oocytes studied with the Y512A and Y512S mutants, the wild-type EC50 value for cAMP was 37 ± 5 μM (mean ± S.E., n = 2), rather than the usual 70 to 80 μM. Therefore, the observed cAMP EC50 values for Y512A and Y512S were normalized to the wild-type value of these batches.
n, number of measurements.