Table 2

Kinetic constants for purified PHM

Treatment GroupKM2-aVmax2-aTurnover2-b
μM pmol/ng/min pmol/pmol/s
Control11.6  ± 0.86.6  ± 0.34.2
Dexamethasone10.8  ± 0.77.4  ± 0.44.7
Disulfiram12.0  ± 0.510.4  ± 0.42-c 6.62-c
Dexamethasone + disulfiram11.3  ± 0.39.7  ± 0.42-c 6.12-c

  • ↵2-a S.E.M. is given for average of four independent analyses.

  • ↵2-b Quantification of PHM catalytic core is based onM r = 38,000.

  • ↵2-c Significantly different from control (p ≤ .01).