Distribution of PDE4 subtypes in human neutrophils and monocytes
| Cell | PDE4 Subtype mRNA | |||
|---|---|---|---|---|
| A | B | C | D | |
| Neutrophil | <0.004 | 70.174 ± 4.336 | <0.002 | 0.192 ± 0.069 |
| (<0.1%) | (99.7%) | (<0.1%) | (0.3%) | |
| Monocyte | 0.970 ± 0.252 | 5.393 ± 0.290 | 0.008 ± 0.002 | 0.352 ± 0.091 |
| (14.4%) | (80.2%) | (0.1%) | (5.2%) | |
| LPS-monocyte | 1.322 ± 0.483 | 35.382 ± 1.432 | 0.005 ± 0.002 | 0.322 ± 0.038 |
| (3.5%) | (95.5%) | (<0.1%) | (0.9%) | |
Numbers are mean ± S.E.M. in amol/fmol β-actin (neutrophil,n = 6) or glyceraldehyde phosphate dehydrogenase (monocytes, n = 3) from separate RNA preparations. Percentages in parentheses are those of each subtype in total PDE4 (A + B + C + D). Because the numbers were obtained by using an identical amount of RNA per reaction tube, the absolute levels of PDE4 subtypes can be compared between the two different cell types. Experimental details are described in Materials and Methods.