Mutations that resulted in no expression of RFC1 or expression of incomplete RFC1 polypeptides
| Cell Line | MTX IC50 (Fold Increase Relative to L1210 Cells) | MTX Influx (% of L1210 Cells) | RFC1 mRNA Levels Relative to L1210 Cells | Nucleotide Change | RFC1 Polypeptide |
|---|---|---|---|---|---|
| D10 | 32 | 1.5 | 1.0 | 3 ATG→ATA | No polypeptide |
| C6 | 220 | 1.0 | 0.27 | 75 TGG→TGA | First 24 aa |
| D15 | 12 | 12 | 1.51 | 315TGG→TGA | First 104 aa |
| D2 | 73 | 2.0 | 0.62 | 1354CAG→TAG | First 451 aa |
| D8 | 290 | 0.5 | 0.50 | Deletion | Deletion |
| D12 | 230 | 0.9 | 0.89 | Deletion | Deletion |
aa, amino acids.