Cell Lines | MTX IC50 (Fold Increase2-a) | MTX Influx (% of L1210 Cells) | RFC1 mRNA Levels2-b | Nucleotide Change | Amino Acid Change |
---|---|---|---|---|---|
Glycine and proline | |||||
D17 | 150 | 1 | 0.90 | 131GGG→GAG | 44 Gly→Glu |
C12 | 210 | 1.1 | 0.67 | 131GGG→GAG | 44 Gly→Glu |
508CAG→TAG | 170 Gln→Stop | ||||
C17 | 37 | 8.9 | 0.98 | 481GGA→AGA | 161 Gly→Arg |
C7 | 170 | 0.8 | 0.24 | 506GGC→GAC | 169 Gly→Asp |
D7 | 77 | 1.2 | 0.56 | 839GGC→GAC | 280 Gly→Asp |
C3 | 500 | 0.4 | 0.72 | 908GGC→GAC | 303 Gly→Asp |
C4 | 280 | 1.2 | 1.1 | 908GGC→GAC | 303 Gly→Asp |
481GGA→AGA | 161 Gly→Arg | ||||
C10 | 170 | 0.7 | 0.78 | 962GGC→GAC | 321 Gly→Asp |
D6 | 350 | 0.6 | 0.57 | 1181GGG→GAG | 394 Gly→Glu |
C9 | 26 | 2.2 | 0.77 | 202CCG→TCG | 68 Pro→Ser |
C13 | 42 | 3.6 | 0.88 | 202CCG→TCG | 68 Pro→Ser |
D16 | 15 | 3.8 | 0.77 | 202CCG→TCG | 68 Pro→Ser |
Arginine and glutamate | |||||
C2 | 87 | 1.5 | 0.66 | 392CGC→CAC | 131 Arg→His |
C1 | 190 | 0.9 | 0.86 | 464CGG→CAG | 155 Arg→Gln |
D19 | 18 | 4 | 0.98 | 757CGC→TGC & | 253 Arg→Cys |
360ATG→ATA | 120 Met→Ile | ||||
D11 | 270 | 0.8 | 0.48 | 1097CGT→CAT | 366 Arg→His |
C8 | 19 | 8 | 1.2 | 133GAA→AAA | 45 Glu→Lys |
332GGC→GAC | 111 Gly→Asp | ||||
D13 | 230 | 3.8 | 0.58 | 133GAA→AAA | 45 Glu→Lys |
Serine and alanine | |||||
D9 | 330 | 0.7 | 0.82 | 497TCT→TTT | 166 Ser→Phe |
D1 | 53 | 2.1 | 0.90 | 497TCT→TTT | 166 Ser→Phe |
1199GCT→GTT | 400 Ala→Val | ||||
D4 | 270 | 0.6 | 0.67 | 950TCC→TTC | 317 Ser→Phe |
919GCC→GTC | 307 Ala→Val | ||||
C15 | 47 | 2.1 | 0.93 | 1154TCT→TTT | 385 Ser→Phe |
C11 | 39 | 5.7 | 0.42 | 1126GCC→ACC | 376 Ala→Thr |