Drug | JN134 top1-1 | JN394 top1─ | JN394 top2-4 | |||
---|---|---|---|---|---|---|
pEMBLyex2-a | pEZ-hTOP12-a | pEMBLyex2-b | pEZ-hTOP12-b | 25 °C2-b | 30 °C2-b | |
MEN10746 | 2.30 | 0.70 | 4.2 | 1.9 | 25 | >50 |
MEN10749 | >10 | 5.90 | >10 | >10 | >50 | >50 |
MEN10733 | >10 | >10 | >50 | >50 | >50 | >50 |
MEN10732 | >10 | >10 | >50 | >50 | N.D.2-c | N.D. |
Idarubicin | 0.50 | 0.22 | 1.15 | 0.7 | 14 | >50 |
Doxorubicin | 6.2 | 3.6 | N.D. | N.D. | 17 | >50 |
Camptothecin | >10 | 0.11 | 32 | 0.28 | N.D. | N.D. |
Values are means of two to three independent determinations and indicate drug concentrations inhibiting 90% of cell colony formation. Note that drug treatments were for 16 h in the case of the JN134top1-1 and JN394top1─ strains, and for 24 h in the case of the JN394top2-4 strain.