Table 2

IC50 values (M) for peptides in competition for125I-labeled salmon CT binding to COS-7 cells cotransfected with 100 ng of hCTRI1− and 100 ng of RAMP (n = 3)

Receptor AloneRAMP1RAMP2RAMP3
Competing peptide
 Rat amylin5.37  ± 2.84 × 10−7 1.72  ± 0.73 × 10−7 8.78  ± 1.96 × 10−8 1.96  ± 0.95 × 10−7
 Salmon CT9.33  ± 6.08 × 10−10 2.24  ± 0.30 × 10−10 1.34  ± 0.46 × 10−10 3.17  ± 0.35 × 10−10
 Human CT1.55  ± 0.23 × 10−8 2.95  ± 0.52 × 10−8 8.27  ± 1.57 × 10−9 1.97  ± 1.06 × 10−8
 Human CGRPα>10−6 >10−6 >10−6 10−6