Table 1

Semiquantitative description^1-a of cellular distribution of DAT immunoreactivity in COS cells transiently expressing each of the 29 Phe→Ala mutants

Mutant^1-b	Perinuclear	Plasma Membrane	Mutant	Perinuclear	Plasma Membrane
WT	+	++	7F357A	++	++
1F69A	+	+++	7F361A	+	+++
1F76A	+	+++	7F364A	++	+
1F86A	+++	+	7F366A	+	+
2F98A	++	+	F390A	++	+
2F105A	+	+++	8F410A	+	++
2F114A	+	++	8F411A	++	+
3F143A	+	++	9F447A	+	++
3F150A	+	+++	9F456A	+	++
3F154A	+	++	9F461A	+	++
3F155A	+	++	F477A	+	++
4F252A	+	+++	10F485A	+	++
6F319A	+	+++	10F497A	+	++
6F325A	+	++	11F530A	+	++
6F331A	+	++	F542A	+	++

Apparent perinuclear and plasma membrane staining was rated by an observer unaware of the mutants using a quantitative 1 to 4 “+” scale. Number of plus signs represents a fraction of the DAT protein. For example, the WT has three plus signs; there is one inPerinuclear and two in Plasma Membrane. This means that approximately one third of the protein is at perinuclear location and two thirds of the protein are in the plasma membrane. Total number of plus signs for each mutant represents relative intensity of immunostaining, compared with WT: the greater the number, the more intense the immunostaining.

↵1-a This description mainly shows expression pattern differences of the mutants relative to WT.
↵1-b Italic, F conserved in other Na⁺/Cl⁻-dependent neurotransmitter transporters.