Table 3

Pharmacological categorization of the 29 Phe → Ala DAT mutants based on [3H]dopamine uptake and [3H]CFT binding affinities

Cocaine Analog Affinity3-aDopamine Affinity3-a
DecreaseIncreaseNormal
Decrease7F364A 1F76A
2F98A
7F361A
F390A
Increase
Normal3F155A8F410A and  19 others
1F69A 6F331A 11F530A

This categorization is a summary of data and analyses presented in Figs. 5, A and C, and 8.

    • ↵3-a A change in K D orK m value of at least 3-fold magnitude compared with the WT.

    • Bold indicates mutation selectively affects cocaine analog binding affinity and/or the K D/K m ratio is ≥3.