Liver microsomal FMO activities of rats pretreated with LPS and LPS plus NOS inhibitors
| Substrates | Groups | CON | LPS | LPS + NNA | LPS + AG | NNA | AG |
|---|---|---|---|---|---|---|---|
| TMA | Sd /24 h | 1.41 ± 0.15 | 1.04 ± 0.111-a | 1.61 ± 0.17c | 1.64 ± 0.29c | 1.56 ± 0.42 | 1.59 ± 0.26 |
| (1.34 ± 0.41) | (0.94 ± 0.18)b | (1.53 ± 0.32)c | (1.52 ± 0.44)d | (1.49 ± 0.33) | (1.47 ± 0.61) | ||
| Rd /6 h | 1.59 ± 0.22 | 1.42 ± 0.39 | 2.54 ± 0.41c | 2.28 ± 0.21c | 1.41 ± 0.22 | 1.59 ± 0.36 | |
| (1.52 ± 0.29) | (1.33 ± 0.31) | (2.50 ± 0.34)c | (2.19 ± 0.25)c | (1.37 ± 0.15) | (1.55 ± 0.31) | ||
| Rd /24 h | 1.74 ± 0.31 | 1.29 ± 0.161-a | 2.52 ± 0.31c | 2.68 ± 0.20c | 1.39 ± 0.49 | 1.72 ± 0.29 | |
| (1.75 ± 0.24) | (1.28 ± 0.24)1-a | (2.45 ± 0.29)c | (2.61 ± 0.19)c | (1.36 ± 0.31) | (1.74 ± 0.35) | ||
| DMA | Sd /24 h | 2.39 ± 0.59 | 1.62 ± 0.151-a | 2.62 ± 0.27c | 2.91 ± 0.33c | 2.88 ± 0.76 | 3.16 ± 0.82 |
| (1.64 ± 0.23) | (1.19 ± 0.32)b | (1.84 ± 0.22)c | (1.91 ± 0.39)c | (1.96 ± 0.55) | (1.89 ± 0.63) | ||
| Rd /6 h | 2.91 ± 0.49 | 2.85 ± 0.31 | 4.40 ± 0.71c | 4.01 ± 0.33c | 3.05 ± 0.29 | 3.21 ± 0.40 | |
| (2.11 ± 0.34) | (2.12 ± 0.26) | (2.89 ± 0.31)c | (2.74 ± 0.19)c | (2.13 ± 0.33) | (1.99 ± 0.27) | ||
| Rd /24 h | 3.09 ± 0.35 | 2.20 ± 0.29b | 4.21 ± 0.31c | 3.84 ± 0.25c | 2.71 ± 0.21 | 2.84 ± 0.39 | |
| (2.27 ± 0.28) | (1.45 ± 0.11)b | (3.29 ± 0.39)c | (2.81 ± 0.39)c | (1.88 ± 0.21) | (2.11 ± 0.33) | ||
| IMP | Sd /24 h | 3.45 ± 0.46 | 1.78 ± 0.291-a | 2.49 ± 0.34c | 2.60 ± 0.42c | 3.32 ± 0.49 | 3.13 ± 0.81 |
| (1.59 ± 0.26) | (0.78 ± 0.32)1-a | (1.28 ± 0.17)d | (1.44 ± 0.38)c | (1.71 ± 0.55) | (2.22 ± 0.41) | ||
| Rd /6 h | 2.89 ± 0.38 | 1.84 ± 0.31b | 3.04 ± 0.51c | 2.72 ± 0.64d | 2.89 ± 0.61 | 2.71 ± 0.39 | |
| (1.62 ± 0.34) | (1.11 ± 0.19)b | (2.49 ± 0.35)c | (2.14 ± 0.29)d | (1.79 ± 0.44) | (1.61 ± 0.51) | ||
| Rd /24 h | 2.91 ± 0.62 | 1.99 ± 0.29b | 2.83 ± 0.39d | 2.68 ± 0.46d | 2.32 ± 0.61 | 2.14 ± 0.66b | |
| (1.76 ± 0.31) | (1.38 ± 0.26)b | (2.17 ± 0.35)c | (1.92 ± 0.40)d | (1.61 ± 0.21) | (1.49 ± 0.32) |
Before and after bubbling with CO gas, the liver microsomal FMO activities were measured by determining rate of NADPH oxidation at 340 nm on addition of various FMO substrates as described inMaterials and Methods. Values represent mean ± S.D. obtained from triplicate measurements with liver microsomes obtained from four or five individual rats in each treatment group.
Abbreviations: Sd/24 h, single dose saline or inhibitors and sacrificed at 24 h; Rd/6 h, 0 and 4 h repeat doses of saline or inhibitors and sacrificed at 6 h (early time point); Rd/24 h, every 4 h repeat doses of saline or inhibitors and sacrificed at 24 h.
↵1-a p < .01 andb p < .05 indicate significant suppression of FMO activities when compared with those obtained from control rat liver microsomes, and c p < .01 andd p < .05 indicate significant restoration toward normal level when compared with those of the respective LPS-treated groups. Values in parentheses indicate FMO activities determined after CO-bubbling.