Additions to Reaction Mixtures | EROD Activity in Aliquots Taken At: | k (min−1) | |
---|---|---|---|
10 min | 30 min | ||
nmol/min/mg | |||
TCB | 2.6 ± 0.5 | 2.4 ± 0.5* | 0.003 |
TCB + NADPH | 2.0 ± 0.5 | 0.9 ± 0.3 | 0.038 |
TCB + NADPH + catalase | 2.1 ± 0.5 | 0.9 ± 0.2 | 0.037 |
H2O2 + TCB + NADPH | 0.8 ± 0.4 | 0.2 ± 0.1* | 0.080 |
H2O2 + TCB + NADPH + catalase | 1.9 ± 0.6 | 0.8 ± 0.2 | 0.040 |
Incubation conditions and assay technique were as described in Table 2, except for the additions of catalase (800 U) and H2O2(1 mM). Data are the means ± S.D. from three pools of microsomes. Rate constants are as in Table 2.
∗, Significantly different from TCB + NADPH (p < .05, Dunnett’s test).