Table 1

Effect of leucine mutations on the rate of internalization of the125I-hCG-rLHR complex

ReceptorT1/2of Internalization
min
rLHR-wt108  ± 7
rLHR-L613,614,615,616A42  ± 41-150
rLHR-L613,614A16  ± 21-150
rLHR-L615,616A76  ± 51-150
rLHR-L613A33  ± 141-150
rLHR-L614A49  ± 81-150

Cells were transiently transfected with the indicated constructs. TheT1/2 values for internalization of the125I-hCG-rLHR complexes were measured as described inMaterials and Methods.

  • Each value represents the mean ± S.E.M. of three to six independent transfections.

  • ↵1-150  p < .05 compared with rLHR-wt.