Table 3

Structure of DNA-RNA hybrid duplex formed by E. coli 5S ribosomal RNA or its fragments and complementary undecadeoxyribonucleotides and efficacy of RNase H-mediated RNA cleavage

Duplex^3-a	Structure	Nuclear Extract	Relative RNase H Cleavage (%, 15 min)
Duplex^3-a	Structure	Nuclear Extract	X = dG	X = dG^s
V (X = dG)	`15 25`	E. coli RNase H	100	13 ± 1
	`{5′..UGGCGGCCGUAGCGCGGUGGUCCCACCUGA..3′`	Molt4	76 ± 2	8 ± 1
VI (X = dG^S)	`3′-TCGCXCCACCA-5′`	CEM	72 ± 4	6 ± 1
		NALM6	58 ± 2	6 ± 1
		HeLa	89 ± 1	19 ± 2
VII (X = dG)	`{ 5′..UAGCGCGGUGGUC..3′`	E. coli RNase H	100	54 ± 6
	`3′-TCGCXCCACCA-5′`	CEM	81 ± 2	69 ± 2
VIII (X = dG^S)		NALM6	81 ± 2	69 ± 3
		P12	83 ± 1	66 ± 2
		HeLa	74 ± 4	74 ± 3

↵3-a In all experiments duplex concentrationC_O, 0.2 × 10⁻⁵ M.