Duplex3-a | Structure | Nuclear Extract | Relative RNase H Cleavage (%, 15 min) | |
---|---|---|---|---|
X = dG | X = dGs | |||
V (X = dG) | 15 25 | E. coli RNase H | 100 | 13 ± 1 |
{5′..UGGCGGCCGUAGCGCGGUGGUCCCACCUGA..3′ | Molt4 | 76 ± 2 | 8 ± 1 | |
VI (X = dGS) | 3′-TCGCXCCACCA-5′ | CEM | 72 ± 4 | 6 ± 1 |
NALM6 | 58 ± 2 | 6 ± 1 | ||
HeLa | 89 ± 1 | 19 ± 2 | ||
VII (X = dG) | { 5′..UAGCGCGGUGGUC..3′ | E. coli RNase H | 100 | 54 ± 6 |
3′-TCGCXCCACCA-5′ | CEM | 81 ± 2 | 69 ± 2 | |
VIII (X = dGS) | NALM6 | 81 ± 2 | 69 ± 3 | |
P12 | 83 ± 1 | 66 ± 2 | ||
HeLa | 74 ± 4 | 74 ± 3 |
↵3-a In all experiments duplex concentrationCO, 0.2 × 10−5 M.