Table 1

GSNO/DMNQ-evoked protection was attenuated by glutathione depletion

DNA FragmentationLDH Release
−BSO+BSO (500 μM)−BSO+BSO (500 μM)
%
Control9  ± 214  ± 36  ± 28  ± 2
GSNO (0.25 mM)24  ± 526  ± 316  ± 340  ± 41-c
DMNQ (10 μM)13  ± 220  ± 25  ± 113  ± 4
GSNO (0.25 mM) + DMNQ (10 μM)17  ± 21-a 25  ± 27  ± 01-d 45  ± 31-b

MC were preincubated for 24 h with or without BSO (500 μM). Then, vehicle (control), GSNO (0.25 mM), DMNQ (10 μM), or the combination of GSNO and DMNQ was added for 24 h. DNA fragmentation was quantified by the diphenylamine assay, and necrosis was measured by LDH release. For details, see Experimental Procedures. Data are mean values (±S.D.) of more than four separate experiments.

  • ↵1-a Significant versus GSNO without BSO (DNA fragmentation).

  • ↵1-b Significant versus GSNO/DMNQ without BSO (LDH release).

  • ↵1-c Significant versus GSNO without BSO (LDH release).

  • ↵1-d Significant versus GSNO without BSO (LDH release).