α Subunit (no. of oocytes) | EC50 | nH |
---|---|---|
μM | ||
Wild type (5) | 31.8 ± 3.4 | 1.32 ± 0.16 |
H101F (3) | 60.3 ± 6.9 | 0.96 ± 0.11 |
H101Y (4) | 42.5 ± 3.0 | 1.33 ± 0.12 |
H101K (7) | 56.6 ± 8.8 | 1.61 ± 0.43 |
H101E (4) | 66.9 ± 3.7 | 1.04 ± 0.06 |
H101Q (3) | 51.4 ± 3.0 | 1.28 ± 0.10 |
cRNAs encoding the wild-type or mutated α1 subunits were coexpressed with the β2 and γ2 subunits in X. laevis oocytes, and currents in response to bath applied GABA were recorded.