Table 3

The binding of atropine analogs to wild-type and Tyr381 mutant M1 mAChRs

graphic

  • Values are mean ± range from two experiments (a) or mean ± S.E. from three experiments. The competing radioligand was [3H]QNB. The Hill coefficients ranged from 0.8 to 1.2 with the exception of NMS and benzilyltropine, which gave Hill coefficients of 0.7 or less.

  • 3-b The results shown are the values for the high-affinity site obtained from fitting the data to a two-site model of ligand binding (see Exceptional Procedures).