Cell line | TS level | RNA concentration | Ratio |
---|---|---|---|
pmol/mg of protein | |||
RJK88.13/Pro303 | 2.6 | 12 | 4.6 |
RJK88.13/Leu303 | 2.7 | 118 | 44 |
RJK88.13/Asp303 | 3.1 | 18 | 5.8 |
HCT15 | 4.0 | 13 | 3.3 |
HCT15/200 | 12.6 | 769 | 61 |
Enzyme levels were determined by the FdUMP binding assay. RNA concentrations, in arbitrary units, were determined by Northern blotting and with the use of a PhosphorImager.