Table 1

Effect of 8-bromo-cAMP and PGE2 on levels of cyclin D1 after stimulation with thrombin (0.3 U/ml) for 20 hours (n = 3)

InhibitorMitogenCyclin D1 protein1-a Mean ± S.E.M.
20  ± 12
Thrombin100
8-bromo-cAMP (300 μM)18  ± 4
8-bromo-cAMP (300 μM)Thrombin51  ± 71-b 1-c
PGE2 (1 μM)5  ± 2
PGE2 (1 μM)Thrombin8  ± 11-b

  • ↵1-a Densitometry-determined levels have been normalized to the level of cyclin D1 in thrombin-stimulated cells (100%) in individual experiments.

  • ↵1-b P < 0.05 vs corresponding levels in absence of inhibitor.

  • ↵1-c P < 0.05 vs corresponding levels in absence of thrombin.