Expression of the α1a-AR, α1b-AR, and their constitutively active mutants A271E and A293E
| Receptor | [125I]HEAT | [3H]Prazosin | ||
|---|---|---|---|---|
| Kd | Bmax | Kd | Bmax | |
| pM | pmol/mg | pM | pmol/mg | |
| α1a-AR | 95 ± 19.8 | 4.3 ± 0.22 | 427 ± 63.6 | 3.4 ± 1.03 |
| A271E | 178 ± 44.8 | 3.4 ± 0.38 | 567 ± 105.4 | 2.7 ± 0.98 |
| α1b-AR | 132 ± 12.7 | 3.3 ± 0.85 | 224 ± 24.0 | 2.3 ± 0.72 |
| A293E | 179 ± 71.15 | 2.1 ± 0.36 | 374 ± 60.7 | 1.7 ± 0.55 |
[125I]HEAT and [3H]prazosin saturation-binding parameters were determined as described in Experimental Procedures. Values are means ± S.E. of three experiments, each performed in triplicate.