NIF and anti-CD11a mAb inhibit PMA-induced aggregation of JY lymphoblastoid cells
| Treatment | Aggregation Scores | ||
|---|---|---|---|
| Expt. 1 | Expt. 2 | Expt. 3 | |
| Basal | 0 | 1+ | 0 |
| PMA | 3+ | 3+ | 4+ |
| PMA + anti-CD11a | 1+ | 0 | 1+ |
| PMA + anti-CD11b | 4+ | 3+ | 4+ |
| PMA + anti-CD18 | 1+ | 1+ | 0 |
| PMA + anti-ICAM-1 | 0 | 0 | 1+ |
| PMA + anti-HLA | 3+ | 3+ | 3+ |
| PMA + NIF | 1+ | 1+ | 1+ |
JY cells were stimulated with PMA (200 ng/ml) for 18 h and the degree of aggregation was scored as described in Materials and Methods. MAbs (10 μg/ml) or NIF (1 μM) were added. Aggregation scores of JY cells from five separate fields within 1-μl well were obtained. Values represent an average of aggregation scores from triplicate microtiter wells.