Sites for common DNA binding factors in the mouse α1BAR promoter
| Factor/Site | Occurrence | Location |
|---|---|---|
| AP-1 | >5 | NR |
| AP-2 | >5 | NR |
| CACCC site | 1 | −615 |
| CCAAT-box | 2 | −1150, −2991 |
| C/EBP | 5 | −656, −1178, −1569, −1688, −2884 |
| CRE | 1 | −441 |
| E box | 1 | −770 |
| GC box | 1 | −447 |
| HIF-1 | 1 | −168 |
| HNF-5 | >5 | NR |
| LBP-1 | >5 | NR |
| M-CAT | 1 | −7371-a |
| NF-1 | 2 | −532,1-b −5601-b |
| NF-E1 | 2 | −1538, −3189 |
| NF-κB | 1 | −795 |
| Spl | >5 | NR |
Sequence analysis of our 3422-bp mouse α1BAR gene promoter fragment revealed the presence of multiple consensus sites on the sense strand that are specific for binding of various trans-activating factors. Of these, several common factors/sites are listed. Sites conserved between the mouse and rat promoters are italicized, and sites found only in the mouse are boldfaced. The location of elements that occur more than 5 times are not reported (NR). Locations reported are relative to the adenosine of the initial ATG codon.
↵1-a As reported in the rat promoter (Gao et al., 1995), the mouse promoter contains a 1-bp mismatch (CATGGCT) of the consensus for the cardiac factor M-CAT (CATNC[C/T][T/A]).
↵1-b These two binding domains (TGGCT and TGGCG), which have been shown to facilitate binding of NF1 in the rat (Gao et al., 1996), represent half of the reported consensus sequence motif for NF1 (MTGGNNNNNNGCCA) (Shaul et al., 1986).