Ligand-binding characteristics of wild-type and CAM β2-adrenoceptor-GFP constructs
| Construct | Kd | Bmax |
|---|---|---|
| nM | pmol/mg membrane protein | |
| β2-adrenoceptor | 0.62 ± 0.26 | 4.1 ± 0.9 |
| Wild type β2-adrenoceptor-GFP | 0.75 ± 0.17 | 8.7 ± 0.3 |
| CAM β2-adrenoceptor-GFP | 0.36 ± 0.13 | 1.0 ± 0.2 |
Data represent means ± S.E. from three independent experiments using [3H]DHA as a radioligand.