PMEG | PMEDAP | PMEA | |||||||
---|---|---|---|---|---|---|---|---|---|
[PMEG] | [PMEGp] | [PMEGpp] | [PMEDAP] | [PMEDAPp] | [PMEDAPpp] | [PMEA] | [PMEAp] | [PMEApp] | |
μM | |||||||||
1 | 0.12 ± 0.00 | 0.13 ± 0.03 | 0.48 ± 0.10 | 0.46 ± 0.06 | 0.06 ± 0.01 | 0.15 ± 0.02 | 0.35 ± 0.05 | 0.09 ± 0.02 | 0.11 ± 0.00 |
3 | 0.32 ± 0.04 | 0.61 ± 0.09 | 2.1 ± 0.3 | 1.6 ± 0.2 | 0.25 ± 0.04 | 0.62 ± 0.10 | 1.3 ± 0.2 | 0.43 ± 0.04 | 0.50 ± 0.08 |
10 | 0.76 ± 0.06 | 2.0 ± 0.4 | 8.0 ± 1.0 | 5.8 ± 0.4 | 0.87 ± 0.10 | 3.0 ± 0.6 | 4.5 ± 0.7 | 1.8 ± 0.3 | 2.2 ± 0.7 |
30 | 3.9 ± 0.3 | 7.0 ± 0.9 | 27.5 ± 2.2 | 24.6 ± 3.0 | 4.1 ± 0.5 | 11.6 ± 2.4 | 19.7 ± 1.7 | 6.8 ± 1.0 | 9.3 ± 1.9 |
100 | 9.6 ± 1.3 | 19.3 ± 2.1 | 91.5 ± 7.5 | 71.3 ± 8.5 | 15.3 ± 2.8 | 44.0 ± 6.3 | 54.7 ± 4.8 | 27.0 ± 3.7 | 36.9 ± 3.4 |
Exponentially growing cells were incubated with the indicated concentrations of nucleotide analogs for 24 h at 37°C and extracted. Intracellular concentrations of nucleotide analogs and their mono- and diphosphates were determined as described in Materials and Methods. Results are means of two separate experiments.