Incorporation efficiency of PMEDAPpp and PMEApp by replicative DNA polymerases with a running-start primer extension assay
| dATP | PMEDAPpp | PMEApp | ||||||
|---|---|---|---|---|---|---|---|---|
| Vmax | Km | Vmax | Km | fINS(%)3-a | Vmax | Km | fINS(%)3-a | |
| pmol/min/mg | nM | pmol/min/mg | nM | pmol/min/mg | nM | |||
| Pol α | 3.3 ± 0.2 | 21 ± 3 | 0.96 ± 0.05 | 238 ± 12 | 2.6 | 0.83 ± 0.08 | 1510 ± 230 | 0.35 |
| Pol δ | 13.8 ± 0.9 | 4.0 ± 0.6 | 12.0 ± 0.5 | 17 ± 2 | 20.5 | 12.5 ± 0.50 | 67 ± 8 | 5.4 |
| Pol ε | 0.56 ± 0.02 | 15 ± 1 | 0.58 ± 0.02 | 370 ± 16 | 4.2 | 0.42 ± 0.03 | 1960 ± 150 | 0.57 |
↵3-a See Table 2.