Mice housed in Amsterdam
| mdr1a (FVB × 129/Ola) n = 3/genotype | mdr1a (FVB) n = 5/genotype | mdr1b (FVB) n = 5/genotype | mdr1a/1b (FVB) n = 5/genotype | |||||
|---|---|---|---|---|---|---|---|---|
| (+/+) | (−/−) | (+/+) | (−/−) | (+/+) | (−/−) | (+/+) | (−/−) | |
| Male | ||||||||
| CYP3A | 1.0 ± 0.50 | 3.01 ± 0.48 | 1.0 ± 0.10 | 2.02 ± 0.23*** | 1.0 ± 0.10 | 1.47 ± 0.14*** | 1.0 ± 0.39 | 3.21 ± 0.49*** |
| CYP1A | 1.0 ± 0.88 | 2.54 ± 0.88 | 1.0 ± 0.17 | 1.72 ± 0.04*** | 1.0 ± 0.17 | 1.38 ± 0.333-150 | 1.0 ± 0.2 | 3.96 ± 1.62*** |
| CYP2B | 1.0 ± 0.62 | 0.82 ± 0.65 | 1.0 ± 0.09 | 3.37 ± 0.86** | 1.0 ± 0.09 | 2.53 ± 1.013-150 | 1.0 ± 0.11 | 8.28 ± 1.62*** |
| P450-R | 1.0 ± 0.38 | 1.79 ± 0.16** | 1.0 ± 0.38 | 2.16 ± 0.62 | 1.0 ± 0.7 | 2.72 ± 0.44** | ||
| CYP2D | 1.0 ± 0.37 | 3.25 ± 0.37*** | ||||||
| CYP4A | 1.0 ± 0.85 | 4.19 ± 0.85*** | ||||||
| Female | ||||||||
| CYP3A | 1.0 ± 0.42 | 1.75 ± 0.253-150 | 1.0 ± 0.42 | 4.50 ± 0.74*** | ||||
| CYP1A | 1.0 ± 0.65 | 1.58 ± 0.16 | 1.0 ± 0.29 | 5.80 ± 2.233-150 | ||||
| CYP2B | 1.0 ± 1.27 | 3.26 ± 0.39 | 1.0 ± 0.02 | 11.5 ± 2.98** | ||||
| P450-R | 1.0 ± 0.07 | 2.56 ± 0.35** | ||||||
| CYP2D | 1.0 ± 0.19 | 3.51 ± 1.173-150 | ||||||
| CYP4A | 1.0 ± 0.8 | 2.30 ± 0.84 | ||||||
Liver microsomes were analyzed by immunoblot with specific P450 antibodies and the bands were quantified by densitometry. The values from the (+/+) mice were assigned the number 1.0. Data are shown as mean ± S.D. of three to five animals per genotype per group with samples analyzed on multiple gels. Statistical difference between the (+/+) and (−/−) mice was assessed by a two-sided Student'st test with P < .05 as the limit of significance.
P-450-R, P450 reductase
↵3-150 P < .05; ** P < .01; *** P < .005.