Mice housed in the United States
| CF1 (untreated) n (+/+) = 5; (+/−) = 7; (−/−) = 10 | mdr1a (FVB) n = 4/genotype | mdr1a/1b (FVB) n = 4/genotype | |||||
|---|---|---|---|---|---|---|---|
| (+/+) | (+/−) | (−/−) | (+/+) | (−/−) | (+/+) | (−/−) | |
| Male | |||||||
| CYP3A | 1.0 ± 0.71 | 1.66 ± 1.15 | 2.08 ± 1.18 | 1.0 ± 0.25 | 0.61 ± 0.254-150 | 1.0 ± 0.18 | 0.79 ± 0.25 |
| CYP1A | 1.0 ± 0.07 | 0.92 ± 0.27 | |||||
| CYP2B | 1.0 ± 0.50 | 0.99 ± 0.51 | 1.0 ± 0.12 | 0.95 ± 0.10 | |||
| mdr1a (FVB) 12 wk n = 2/genotype | mdr1a (FVB) 17 wk n = 3/genotype | mdr1a (FVB) 23 wk n = 3/genotype | ||||
|---|---|---|---|---|---|---|
| (+/+) | (−/−)4-a | (+/+) | (−/−) | (+/+) | (−/−) | |
| Female | ||||||
| CYP3A | 1.0 | 1.23 ± 0.35 | 1.0 ± 0.42 | 2.11 ± 0.134-150 | 1.0 ± 0.51 | 0.76 ± 0.37 |
| CYP1A | 1.0 | 1.08 ± 0.04 | 1.0 ± 0.15 | 1.00 ± 0.23 | 1.0 ± 0.23 | 0.72 ± 0.80 |
| CYP2B | 1.0 | 0.97 ± 0.51 | 1.0 ± 0.09 | 1.92 ± 0.214-150 | 1.0 ± 0.14 | 1.00 ± 0.17 |
Liver microsomes were analyzed by immunoblot with specific P450 antibodies and the bands quantified by densitometry. The values from the (+/+) mice were assigned the number 1.0. Data are shown as mean ± S.D. of three to five animals per genotype per group with samples analyzed on multiple gels. Statistical difference between the (+/+) and (−/−) mice was assessed by a two-sided Student'st test with P < .05 as the limit of significance.