Table 4

Rate constants governing inhibitor complex formation

Ligand Enzyme	FdUMP		AG337
Ligand Enzyme	Wild-type ecTS	P254DecTS	Wild-type ecTS	P254DecTS
k _on (μM⁻¹s⁻¹)	7.3	9.4	19	26
k _off(s⁻¹)	53	230	70	550
K _d(μM)^4-a	7.3	24.5	3.7	21.2

Errors are within 10% of the reported values.

↵4-a Determined from K _d =k _off/k _on.