Competitor Added | Concentration (μM) | % Binding ± S.E.M. | |
---|---|---|---|
1 | – | – | 100 ± 3 |
2 | D3 | 0.05 | 100 ± 14 |
3 | D3 | 0.2 | 89 ± 8 |
4 | D3 | 1 | 64 ± 10 |
5 | D3 | 5 | 43 ± 12 |
6 | D3 | 20 | 38 ± 7 |
7 | D3 | 40 | 31 ± 4 |
8 | C59 | 40 | 96 ± 9 |
The binding of mAb 5C3 (at constant 2 nM) to purified TrkA-ECD immobilized onto ELISA plates was measured in the absence or presence of competitors. Background (<2%) was the OD of wells with all reactants except immobilized TrkA-ECD. Data are averaged from three experiments (n = 1).