Table 1

PCR primers

RGSSpecies Accession No.Primer Sequences 5′ to 3′Expected Product
1RatGCCAACCAGATGGGTCAAAATGTC240 bp
RNU77698ATGTGGGAGTTGGTGTTTTGATCTTC
2MouseAGTAAATATGGGCTGGCTGCATTCA229 bp
MMU67187GCCTCTTGGATATTTTGGGCAATCA
MouseCGGGAGTCTGAGAATGCAAAGTG 1.1kb
MMU67187CTAGTACTAATGATCTGTAGTCCTAAGAAC
RatGCCCCATGCTACATGAGACAG486 bp
Our cloneTGTAGTCCTAAGAAACGTCAGTCAAC
3RatTTTCAAGAAGGTCAAATCGCAGTCCA162 bp
RNU32434GTCAAAGCAGCCTCGGGTGAT
4RatTCAGCTGTGAGGAGTACAAGAAAATCAAA186 bp
RNU32327TTCTGGGCTTCATCAAAACAGGTTATC
5RatAAGTCCCCCATCAAAATGGCAGAGA158 bp
RNU32435CTGGGCCAGGTCAAAGCTGTG
6RatTGGCCGTCCAAGATCTCAAGAAGCAA193 bp
RNU32436GTAGATGTGCTCCTGGGCATCTTCAAAT
7RatTTGGCAGTGGAGGACCTGAAGAAAAG200 bp
RNU32328CAGCTTGTAAATGTGCTCCTGAGCATCT
8RatGCCTGTGAGGAGTTCAAGAAGAC184 bp
RNU32432CCCTGGGCTTGATCAAAACAAGTC
9RatCTGAAGTATGGGGATCAGTCCAAGGTCAA172 bp
RNU32433TGGGTCTGCGCCGCGTCCAAC
10RatTGAAGATTTCAAGAAAACGGAGGACAAG167 bp
RNU32437TTTTGGAACATCAGAGGGTGTGGT
RatGGAAGATCCAGAGGGCGTGAAGAGA503 bp
AA801173 CACACTCCAGTCCTCAAACACAACAAAG
11RatGTCCCCACCCTGGTGGACT160 bp
RNU32438AGCATGTAGATGTGCAGTTGTGCTG
12RatATCGAAATGTTAGAAAGACCAAAGAGGAC 1.1kb
RNU92280ATGGAAAACCCGGACTTGACAGCA
13HumanGGCGGAATTGTTGGATTTGTAAGATGT472 bp
AF030107 GAAACTGTTGTTGGACTGCATAGTTTTCA
14RatGGAGGCCCACAACATCTACCAT 1.1kb
RNU92279GCATCTTCTACCGACAAACTGGAG
16MouseCGAGGAGTTCAAGAAGATCCGATCAG177 bp
MMU67189CTGAGCCACATCGAAGCAACTGGT
MouseTCCGCCACCATCCTCTCCAC512 bp
MMU67189TGTCGTCAGCCCTCAGTCACAG
RatAAAACAGGGATTTCGTACACCACTGATAA423 bp
ESTAA817864 CGACAACTGAGCAAAGGAGAGAAG
GAIPRatCCACCCCACATGAGGCTGAGAAA627 bp
AF068136 GCCCCCTGGAGTAACAGAGAG

PCR primers specific for RGS subtypes were designed to anneal to the indicated sequences. The size of the expected product is also indicated.