Comparison of IC50 values of RPR112378 and RPR115781 after incubation with several sulfhydryl molecules
| Treatment | Incubation time | Tubulin assay IC50 |
|---|---|---|
| h | μM | |
| RPR112378 | ||
| None | 1 | |
| 10 mM cysteine | 1 | 7.5 |
| 10 mM cysteine | 48 | 10 |
| 400 mM cysteine | 1 | 10 |
| 400 mM cysteine | 24 | 15 |
| 10 mM dithiothreitol | 1 | 15 |
| 10 mM dithiothreitol | 24 | 20 |
| 1 M β-mercaptoethanol | 1 | 20 |
| 10 mM lysine | 1 | 1 |
| RPR115781 | ||
| None | 6 | |
| 1 M β-mercaptoethanol | 1 | 6 |