Measurement of excess unreacted RPR112378 and calculated bound RPR112378 to tubulin
| Tubulin | RPR112378 | Tubulin | RPR112378 | Unreacted RPR112378 | Calculated bound RPR112378 | R = Bound RPR112378/tubulin |
|---|---|---|---|---|---|---|
| μM | μM | nmol | nmol | nmol | ||
| 10 | 0 | 3 | 0 | 0 | 0 | 0 |
| 10 | 3 | 3 | 0.78 | 2.22 | 0.74 | |
| 20 | 3 | 6 | 2.3 | 3.7 | 1.2 | |
| 40 | 3 | 12 | 8 | 4 | 1.33 | |
| 100 | 3 | 30 | 25 | 5 | 1.6 | |
| 20 | 0 | 6 | 0 | 0 | 0 | 0 |
| 20 | 6 | 6 | 2.5 | 3.5 | 0.66 | |
| 80 | 6 | 24 | 17.3 | 6.7 | 1.11 |