Regulation of DNA sequences of the rat HO-1 and PCK gene promoter 5′-flanking regions by OA and Bt2cAMP in transiently transfected rat hepatocyte cultures
| Construct | Fold induction of reporter gene activity | ||
|---|---|---|---|
| OA | Bt2cAMP | OA + Bt2cAMP | |
| pHO-1338 Luc | 2.5 ± 0.2 | 2.5 ± 0.3 | 4.5 ± 0.5* |
| pHO-754 Luc | 3 ± 0.5 | 3.5 ± 1 | 6 ± 0.4* |
| pPCK-2500 CAT | 1 ± 0.2 | 4 ± 0.4** | 2.5 ± 0.2 |
| pGL3prom Luc | 0.1 ± 0.2 | 0.1 ± 0.1 | 0.1 ± 0.2 |
The indicated rat HO-1 or PCK gene sequences were cloned into pGL3Luc or pCAT, as indicated, and the reporter constructs were transiently transfected into primary rat hepatocyte cultures. After 24 h, the transfected cells were treated for 12 h with OA (5 nM), Bt2cAMP (100 μM) or a combination of OA plus Bt2cAMP. The rate of induction in each experiment relative to the control was determined. The values are from three independent experiments (mean ± S.E.). Student's t test for paired values:*indicates significant difference OA versus OA + Bt2cAMP;P ≤ .05; **significant difference OA versus Bt2cAMP, P ≤ .05.