Receptor | Basal IP Levels | Basal IP Levels in the Presence of Prazosin1-a | Prazosin Effect as % of Basal IP Levels | Basal ERK Activity1-b | Basal ERK Levels in the Presence of Prazosin | Prazosin Effect as % of Basal ERK Levels |
---|---|---|---|---|---|---|
cpm | cpm | IOD | IOD | |||
α1B-AR | 587.4 ± 37.2 | 645.6 ± 57.4 | 109.9 | 1.736 ± 0.755 | 2.653 ± 1.074 | 152.8 |
α1D-AR | 1069 ± 163.5 | 876.4 ± 128.9 | 81.981-c | 3.466 ± 0.943 | 1.824 ± 0.728 | 52.61-c |
Each point represents the mean ± S.E. for four (inositol phosphate) or five (ERK) independent experiments.
↵1-a Basal inositol phosphates were measured as described in Materials and Methods.
↵1-b Basal ERK activity is expressed as integrated optical density (IOD) units. IOD values were obtained from densitometer scans of the in gel-kinase assays as described in Materials and Methods.
↵1-c Significantly (P < .5) different from basal activity obtained in the absence of prazosin.