Table 1

Effect of temperature on binding parameters of 5-[125I]iodo-A-85380

Binding ParameterIncubation Temperature
4°C22°C37°C
K d, pM9.9  ± 0.810.6  ± 0.320  ± 2
B max, fmol/mg protein162  ± 5167  ± 5166  ± 6

Rat brain P2 membrane fractions (5 μg of protein) were incubated in a total volume of 0.3 ml with 1 to 500 pM 5-[125I]iodo-A-85380 for 4 h at 22°C or 37°C, or for 24 h at 4°C. Results were analyzed as described in Experimental Procedures. Data represent mean ± S.E. obtained from three to four experiments per incubation temperature. Experiments were performed in quadruplicate.