Effect of PNA antisense treatment on δ-opioid receptor density
| [3H]Naltrindole Saturation Binding | [35S]GTPγS Binding | |||
|---|---|---|---|---|
| Kd | Bmax | SNC80 EC50 | % Maximal Stimulated Binding | |
| nM | mol/mg protein | nM | ||
| Saline-treated (n = 5) | 0.059 ± 0.007 | 44.3 ± 2.8 | 71.5 ± 6.2 | 100.0 ± 5.8 |
| Antisense-treated (n = 5) | 0.055 ± 0.010 | 38.6 ± 2.2 | 85.5 ± 5.4 | 74.8 ± 5.01-a |
| Mismatch-treated (n = 5) | 0.069 ± 0.010 | 43.4 ± 0.9 | 65.8 ± 5.8 | 97.0 ± 8.1 |
Saturation and [35S]GTPγS binding were performed on homogenates of brain hemispheres from saline-, antisense-, and mismatch-treated rats. The data from each rat brain homogenate was analyzed separately. Basal [35S]GTPγS binding was 3240 ± 120, 3230 ± 150, and 3220 ± 120 cpm for the saline-, antisense-, and mismatch-treated groups respectively; there was no significant difference between treatment groups (P > .05). Maximal stimulated binding is defined as the peak increase over basal levels for SNC80-induced [35S]GTPγS binding in brain homogenates prepared from saline-treated animals. Saturation binding and [35S]GTPγS binding were assayed in parallel on the same brain homogenates.
↵1-a Represents a significant difference in comparison to the saline-treated group (P < .05). Data are presented as mean ± S.E.