Brain Region | Total G Protein Activation | |||
---|---|---|---|---|
[32P]AA-GTP | [35S]GTPγS | |||
OD UNITS | % INCREASE | pmol/mg | % INCREASE | |
Cerebellum | 1922 ± 351 | 131.3 ± 3.4 | 0.258 ± 0.051 | 676.0 ± 39.0 |
Hippocampus | 2800 ± 524 | 68.1 ± 5.81-150 | 0.235 ± 0.052 | 252.0 ± 13.01-150 |
Striatum | 2635 ± 435 | 56.4 ± 4.71-150 | 0.358 ± 0.066 | 155.0 ± 5.21-150 |
Amygdala | 1888 ± 478 | 64.2 ± 9.81-150 | 0.271 ± 0.035 | 89.2 ± 11.01-150 |
Hypothalamus | 1304 ± 1161-150 | 89.9 ± 6.11-150 | 0.285 ± 0.062 | 182.0 ± 20.01-150 |
Cannabinoid activation of total G proteins was measured by [32P]AA-GTP photoaffinity labeling and [35S]GTPγS binding with 10 μM WIN 55212-2 in membranes prepared from various rat brain regions. Results are expressed as net OD units from densitometric analysis of 32P autoradiograms and net picomoles per microgram of agonist-stimulated [35S]GTPγS binding, along with percentage of stimulation by agonist in each assay. Data represent the mean ± S.E. from at least three to four separate experiments.
↵1-150 P < .05 (ANOVA) different from cerebellum.