Table 1

Amino acid sequence analysis of tryptic peptides I and II derived from hDPD inactivated with [¹⁴C]BVU in the presence of NADPH

Peptide I		Peptide II		hDPD^1-a
Cycle	Amino acid		Cycle	hDPD^1-a	Amino acid
		pmol			pmol
1	Lys	65.9				Lys⁶⁵⁶
2	Ser	29.6	1	Ser	25.2	Ser
3	Glu	27.5	2	Glu	8.8	Glu
4	Asp	19.9	3	Asp	10.3	Asp
5	Ser	14.7	4	Ser	6.0	Ser
6	Gly	24.4	5	Gly	10.6	Gly
7	Ala	21.5	6	Ala	5.9	Ala
8	Asp	11.3	7	Asp	5.8	Asp
9	Ala	24.6	8	Ala	6.4	Ala
10	Leu	14.2	9	Leu	5.3	Leu
11	Glu	9.9	10	Glu	2.6	Glu
12	Leu	18.6	11	Leu	7.1	Leu
13	Asn	14.5	12	Asn	5.3	Asn
14	Leu	18.7	13	Leu	0.4	Leu
15	Ser	6.9	14	Ser	2.2	Ser
16	X^1-b		15	X^1-b		Cys⁶⁷¹
17	Pro	7.5	16	Pro	2.2	Pro
18	His	0.6	17	His	0.2	His
19	Gly	8.9	18	Gly	5.6	Gly
20	Met	3.6	19	Met	1.3	Met
21	Gly	12.4	20	Gly	6.1	Gly
22	Glu	2.1	21	Glu	1.0	Glu
23	Arg	0.6	22	Arg	0.9	Arg⁶⁷⁸

↵1-a Amino acid sequence of hDPD was taken from Yokota et al. (1994).
↵1-b This residue was not identified. About 90% of radioactivity of radiolabeled peptides I and II was eluted at cycles 16 and 15, respectively, and most of the remainder at next two cycles.