Effects of progesterone and finasteride on the amount of AP in cells and conditioned medium of cerebellar granule cell cultures
| Treatment | AP | |||
|---|---|---|---|---|
| Cells | Medium | |||
| % of control | ng/g protein | % of control | ng/ml medium | |
| Control | 100 ± 9 | 0.60 ± 0.05 | 100 ± 5 | 0.56 ± 0.03 |
| Progesterone | 563 ± 1231-a | 3.40 ± 0.70 | 269 ± 341-a | 1.50 ± 0.19 |
| Finasteride + progesterone | 106 ± 121-b | 0.63 ± 0.07 | 102 ± 141-b | 0.57 ± 0.08 |
| Finasteride | 70 ± 5 | 0.42 ± 0.03 | 68 ± 7 | 0.38 ± 0.04 |
Cultures were incubated for 5 days in the absence or presence of 1 μM progesterone or 1 μM finasteride as indicated, after which the amount of AP in cells and medium was measured by radioimmunoassay. Data are expressed as the percentage of control (solvent), and are means ± S.E. of values from four independent experiments (n = 11 to 16).
↵1-a P < .01 versus solvent.
↵1-b P < .01 versus progesterone.