Receptor binding affinity and calcium mobilizing potency of chemokines for the rat CXCR3 and human CXCR3
| Chemokine | EC50 | IC50 | ||
|---|---|---|---|---|
| rCXCR3, Ca2+ | hCXCR3, Ca2+ | rCXCR3, Bind | hCXCR3, Bind | |
| nM | ||||
| hIP-10 | 2.8 ± 0.3 | 13.5 ± 3.1 | 2.3 ± 0.1 | 2.4 ± 0.4 |
| mIP-10 | 2.2 ± 0.4 | 24.1 ± 2.7 | 3.3 ± 0.6 | 4.1 ± 0.7 |
| hMig | 21.1 ± 1.7 | 43.3 ± 4.9 | >1000 | >1000 |
| mMig | 4.2 ± 0.2 | 9.3 ± 2.1 | 480 ± 47 | 880 ± 68 |
| I-Tac | 13.0 ± 1.9 | 28.9 ± 3.6 | 40 ± 11 | 145 ± 18 |
| m6Ckine | >100 | ≫100 | >1000 | >1000 |
| h6Ckine | ≫100 | ≫100 | >1000 | >1000 |
Values presented are the EC50 (mean ± S.E.) for chemokine-induced calcium mobilization in HEK 293 rCXCR3 and HEK 293 hCXCR3 cells from six concentration-response curves generated in two independent experiments. Binding values are the IC50 values (mean ± S.E.) for three experiments run with duplicate samples for each concentration of chemokine.