Oligonucleotides used for site-directed mutagenesis of mousemdr3
| Amino Acid Mutant (W → F) | Primer Sequence | 5′ Nucleotide |
|---|---|---|
| W44F (F1) | 5′-CCTGTCCAGGAAACCTGCATAAC-3′ | 292 |
| W132F (F2) | 5′-CTGCCAGGCAGAAAAATGAAACC-3′ | 557 |
| W158F (F3) | 5′-CACATCAAAGAAGCCTATCTC-3′ | 634 |
| W208F (F4) | 5′-GGTTAGCTTGAAGCCACGGGT-3′ | 784 |
| W228F (F5) | 5′-CAATATCTTTGCGAAAATACCAGC-3′ | 847 |
| W311F (F6) | 5′-GAAGTCCCATAGAAGAATGCCAACGCGTATGATGCATAG-3′ | 10951-a |
| W694F (F7) and W704F (F8) | 5′-CAAAATAAGGGAATTCAGTTGAATTCAACTTCAGGATCCGGAAAAAGGAAGC-3′ | 2273 |
| W799F (F9) | 5′-GTCATCAAAGAAGCTCACATC-3′ | 2557 |
| W851F (F10) | 5′-GTGTTAGTTGGAAGCCATAGA-3′ | 2714 |
| W1104F (F11) | 5′-GTGCTCGGAGGAACTGGACATTC-3′ | 3473 |
All oligonucleotides are in the antisense orientation. Underlined codons are mutant positions.
↵1-a Additional introduction of theMluI site.