↵2-a Isolated hepatocytes from ADX rats were incubated with 100 nM steroid, and the induction of tyrosine amino transferase (TAT) activity was measured. Results represent fold induction (mean ± S.E. of three experiments performed in duplicate) with respect to the control incubated with vehicle alone (4.0 ± 1.0 μmol of tyrosine × 10⁻⁶ cells × min⁻¹).

↵2-b Glycogen was obtained from the liver of ADX rats after acute treatment with the indicated steroid. Values are expressed as a fold increase (mean ± S.E. for eight rats per group) with respect to controls injected with vehicle (9.1 ± 1.2 mg of glycogen/g of liver).

↵2-c RBAs were calculated from competition curves between the steroid and 5.0 nM [³H]CORT. Thymocyte cytosol homogenized in TEGI buffer was used as a source of GR. Rat plasma was fractionated with solid ammonium sulfate at 30 to 60% of saturation, dissolved in TEGI buffer afterward, dialyzed, and used as a source of albumin-free CBG. Values are expressed in nanomolar and represent the mean ± S.E. of three independent experiments, each one performed in quintuplicate.